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enrichment depletion  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec enrichment depletion
    Enrichment Depletion, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igg+microbeads/Anti-Mouse+IgG1+MicroBeads/us12692511-359-104-108
    Average 95 stars, based on 61 article reviews
    enrichment depletion - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Enterotoxigenic Escherichia coli heat-labile enterotoxin induces cell death and disrupts effector functions in porcine monocytes
    Article Snippet: The PBMCs were subsequently isolated by density gradient centrifugation on lymphoprep (Axis-Shield, Dundee, UK). .. Isolated PBMCs were incubated with mouse anti-porcine CD14 antibodies (clone MIL-2, in-house production) for 40 min. After washing, the cells were incubated with secondary goat-anti-mouse IgG Microbeads (Miltenyi, Bergisch Gladbach, Germany) for another 20 min. .. Subsequently, the cells were diluted in 5 mL of PBS-EDTA + 1% FCS and applied onto an LS column (Miltenyi) to obtain CD14 + cells.

    Article Title: Enterotoxigenic Escherichia coli heat-labile enterotoxin induces cell death and disrupts effector functions in porcine monocytes.
    Article Snippet: The PBMCs were subsequently isolated by density gradient centrifugation on lymphoprep (Axis-Shield, Dundee, UK). .. Isolated PBMCs were incubated with mouse anti-porcine CD14 antibodies (clone MIL-2, in-house production) for 40 min. After washing, the cells were incubated with secondary goat-anti-mouse IgG Microbeads (Miltenyi, Bergisch Gladbach, Germany) for another 20 min. .. Subsequently, the cells were diluted in 5 mL of PBS-EDTA + 1% FCS and applied onto an LS column (Miltenyi) to obtain CD14+ cells.

    Incubation:

    Article Title: Enterotoxigenic Escherichia coli heat-labile enterotoxin induces cell death and disrupts effector functions in porcine monocytes
    Article Snippet: The PBMCs were subsequently isolated by density gradient centrifugation on lymphoprep (Axis-Shield, Dundee, UK). .. Isolated PBMCs were incubated with mouse anti-porcine CD14 antibodies (clone MIL-2, in-house production) for 40 min. After washing, the cells were incubated with secondary goat-anti-mouse IgG Microbeads (Miltenyi, Bergisch Gladbach, Germany) for another 20 min. .. Subsequently, the cells were diluted in 5 mL of PBS-EDTA + 1% FCS and applied onto an LS column (Miltenyi) to obtain CD14 + cells.

    Article Title: Enterotoxigenic Escherichia coli heat-labile enterotoxin induces cell death and disrupts effector functions in porcine monocytes.
    Article Snippet: The PBMCs were subsequently isolated by density gradient centrifugation on lymphoprep (Axis-Shield, Dundee, UK). .. Isolated PBMCs were incubated with mouse anti-porcine CD14 antibodies (clone MIL-2, in-house production) for 40 min. After washing, the cells were incubated with secondary goat-anti-mouse IgG Microbeads (Miltenyi, Bergisch Gladbach, Germany) for another 20 min. .. Subsequently, the cells were diluted in 5 mL of PBS-EDTA + 1% FCS and applied onto an LS column (Miltenyi) to obtain CD14+ cells.

    Article Title: Mesothelioma cell heterogeneity identified by single cell RNA sequencing.
    Article Snippet: The incubated samples were then processed through a MACS LD column (#130-042-901, Miltenyi), and fractions of CD45− cells were collected for further processing. .. Subsequently, these CD45− cells were incubated with rat anti-mouse MSLN antibody (#D233-3, MBL International) for 20 min in the refrigerator, followed by incubation with anti-rat Scientific Reports | (2025) 15:8725 13| https://doi.org/10.1038/s41598-025-92542-3 IgG microbeads (#130-048-502, Miltenyi) for 15 min in the refrigerator. ..

    Article Title: Ursolic acid inhibits the proliferation of triple‑negative breast cancer stem‑like cells through NRF2‑mediated ferroptosis
    Article Snippet: The manufacturer's protocol for the CD24 Microbead Kit (cat. no. 130-095-951; Miltenyi Biotec GmbH) was adhered to for the cultivation of 3×10 7 MDA-MB-231 and BT-549 mammospheres using a primary antibody against CD24. .. Subsequently, the labeled cells were incubated with IgG microbeads (Miltenyi Biotec GmbH) and underwent magnetic separation using MiniMACS columns (Miltenyi Biotec GmbH). .. The purified CD24 − cells were then incubated with CD44 microbeads (cat. no. 130-095-194; Miltenyi Biotec GmbH), washed and subjected to magnetic separation once more.

    Article Title: Ursolic acid inhibits the proliferation of triple‑negative breast cancer stem‑like cells through NRF2‑mediated ferroptosis.
    Article Snippet: The manufacturer's protocol for the CD24 Microbead Kit (cat. no. 130‐095‐951; Miltenyi Biotec GmbH) was adhered to for the cultivation of 3x107 MDA‐MB‐231 and BT‐549 mammospheres using a primary antibody against CD24. .. Subsequently, the labeled cells were incubated with IgG microbeads (Miltenyi Biotec GmbH) and underwent magnetic separation using MiniMACS columns (Miltenyi Biotec GmbH). .. The purified CD24‐ cells were then incubated with CD44 microbeads (cat. no. 130‐095‐194; Miltenyi Biotec GmbH), washed and subjected to magnetic separation once more.

    other:

    Article Title: GPNMB + Gal‐3 + hepatic parenchymal cells promote immunosuppression and hepatocellular carcinogenesis
    Article Snippet: IgG microBeads , Miltenyi Biotec (Bergisch Gladbach, Germany) , 130‐048‐602 , –.

    Labeling:

    Article Title: Novel Approach in DNA vaccine development against porcine circovirus type 2
    Article Snippet: Peripheral blood mononuclear cells (PBMC) were isolated from the buffy-coat fraction of pig blood by using Histopaque-1077 (Sigma-Aldrich, St. Louis, MO) through densitygradient centrifugation (400 g for 30 min). .. SWC3-positive monocytes were enriched from total PBMC by immunomagnetic labeling of cells using the anti-SWC3 porcine pan-myeloid cell marker (CD172a, AbD serotec, Raleigh, NC) and goat-antimouse IgG microbeads (Miltenyi Biotec, Auburn, CA). .. DCs were generated from sorted monocytes for 7 days at 39°C (Carrasco et al., 2001; Paillot et al., 2001) and 5% CO2 in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA), 1X Non-essential amino acids (Invitrogen, Carlsbad, CA), 1X Sodium pyruvate (Invitrogen, Carlsbad, CA), 1X GlutaMAX (Invitrogen, Carlsbad, CA), and 1% HEPES (Invitrogen, Carlsbad, CA), 10 μg/mL Gentamicin (Invitrogen, Carlsbad, CA), 25 ng/ml recombinant pig IL-4 (Cell Sciences, Canton, MA), and 2× recombinant pig GM-CSF (as a gift from Dr.

    Article Title: Ursolic acid inhibits the proliferation of triple‑negative breast cancer stem‑like cells through NRF2‑mediated ferroptosis
    Article Snippet: The manufacturer's protocol for the CD24 Microbead Kit (cat. no. 130-095-951; Miltenyi Biotec GmbH) was adhered to for the cultivation of 3×10 7 MDA-MB-231 and BT-549 mammospheres using a primary antibody against CD24. .. Subsequently, the labeled cells were incubated with IgG microbeads (Miltenyi Biotec GmbH) and underwent magnetic separation using MiniMACS columns (Miltenyi Biotec GmbH). .. The purified CD24 − cells were then incubated with CD44 microbeads (cat. no. 130-095-194; Miltenyi Biotec GmbH), washed and subjected to magnetic separation once more.

    Article Title: Ursolic acid inhibits the proliferation of triple‑negative breast cancer stem‑like cells through NRF2‑mediated ferroptosis.
    Article Snippet: The manufacturer's protocol for the CD24 Microbead Kit (cat. no. 130‐095‐951; Miltenyi Biotec GmbH) was adhered to for the cultivation of 3x107 MDA‐MB‐231 and BT‐549 mammospheres using a primary antibody against CD24. .. Subsequently, the labeled cells were incubated with IgG microbeads (Miltenyi Biotec GmbH) and underwent magnetic separation using MiniMACS columns (Miltenyi Biotec GmbH). .. The purified CD24‐ cells were then incubated with CD44 microbeads (cat. no. 130‐095‐194; Miltenyi Biotec GmbH), washed and subjected to magnetic separation once more.

    Marker:

    Article Title: Novel Approach in DNA vaccine development against porcine circovirus type 2
    Article Snippet: Peripheral blood mononuclear cells (PBMC) were isolated from the buffy-coat fraction of pig blood by using Histopaque-1077 (Sigma-Aldrich, St. Louis, MO) through densitygradient centrifugation (400 g for 30 min). .. SWC3-positive monocytes were enriched from total PBMC by immunomagnetic labeling of cells using the anti-SWC3 porcine pan-myeloid cell marker (CD172a, AbD serotec, Raleigh, NC) and goat-antimouse IgG microbeads (Miltenyi Biotec, Auburn, CA). .. DCs were generated from sorted monocytes for 7 days at 39°C (Carrasco et al., 2001; Paillot et al., 2001) and 5% CO2 in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA), 1X Non-essential amino acids (Invitrogen, Carlsbad, CA), 1X Sodium pyruvate (Invitrogen, Carlsbad, CA), 1X GlutaMAX (Invitrogen, Carlsbad, CA), and 1% HEPES (Invitrogen, Carlsbad, CA), 10 μg/mL Gentamicin (Invitrogen, Carlsbad, CA), 25 ng/ml recombinant pig IL-4 (Cell Sciences, Canton, MA), and 2× recombinant pig GM-CSF (as a gift from Dr.



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